Les publications suivantes ont été réalisées par des scientifiques du Consortium Stop Buruli.

Genetic fine typing of Mycobacterium ulcerans

Different options for the development of a genetic fingerprinting method for M. ulcerans have been evaluated (1, 2, 3). A single nucleotide polymorphisms based typing method has been developed and successfully applied in a field study, demonstrating focal transmission of M. ulcerans (4). The genome, evolution and diversity of M. ulcerans has been reviewed (5).

1. Genomic Diversity and Evolution of Mycobacterium ulcerans Revealed by Next-Generation Sequencing.

Weihong Qi, Michael Käser, Katharina Röltgen, Dorothy Yeboah-Manu, Gerd Pluschke
PloS Pathog., Research article, published September 2009 | PMID: 19806175 | Free article 
 

2. Single nucleotide polymorphisms on the road to strain differentiation in Mycobacterium ulcerans.
Käser M., Hauser J., Pluschke G.
J Clin Microbiol., Research article, published November 2009 | PMID: 19726608 | Free article

3. Lack of insertional-deletional polymorphism in a collection of Mycobacterium ulcerans isolates from Ghanaian Buruli ulcer patients.

Käser M., Gutmann O., Hauser J., Stinear T., Cole S., Yeboah-Manu D., Dernick G., Certa U., Pluschke G.
J Clin Microbiol., Research article, published November 2009 | PMID: 19726605 | Free article

4. Single Nucleotide Polymorphism Typing of Mycobacterium ulcerans Reveals Focal Transmission of Buruli Ulcer in a Highly Endemic Region of Ghana.

Katharina Röltgen, Weihong Qi, Marie-Thérèse Ruf, Ernestina Mensah-Quainoo, Sacha J. Pidot, Torsten Seemann, Timothy P. Stinear, Michael Käser, Dorothy Yeboah-Manu, Gerd Pluschke 

PloS Negl Trop Dis., Research article, published 20 July 2010 | PMID: 20652033 | Free article

5. The genome, evolution and diversity of Mycobacterium ulcerans.
Katharina Röltgen, Timothy P. Stinear, Gerd Pluschke
Infect Genet Evol., Research Article, published 28 January 2012 | PMID: 22306192 | Read abstract

Transmission

Interaction of M. ulcerans with mosquitoes has been studied (11) and possums have been identified as a potential animal reservoir in an Australian BU endemic area (7). M. ulcerans DNA has been traced in the environment (9), but not in fecal samples from BU patients (8). In a study in Benin detection of M. ulcerans in the environment predicted prevalence of BU (12). In addition, BU prevalence and altitude has been studied (6) and current knowledge on the ecology and transmission of BU has been reviewed (11).

6. Application of real-time PCR in Ghana, a Buruli ulcer-endemic country, confirms the presence of Mycobacterium ulcerans in the environment.
Vandelannoote K, Durnez L, Amissah D, Gryseels S, Dodoo A, Yeboah S, Addo P, Eddyani M, Leirs H, Ablordey A, Portaels F.
FEMS Microbiol Lett., Research Letter, published March 2010| PMID: 20146745 | Read abstract

7. A Major Role for Mammals in the Ecology of Mycobacterium ulcerans

Janet A. M. Fyfe, Caroline J. Lavender, Kathrine A. Handasyde, Alistair R. Legione, Carolyn R. O'Brien, Timothy P. Stinear, Sacha J. Pidot, Torsten Seemann, M. Eric Benbow, John R. Wallace, Christina McCowan, and Paul D. R. Johnson

PLoS Negl Trop Dis., Research article, published 10 August 2010 | PMID: 20706592 | Free article

8. Ecology and Transmission of Buruli Ulcer Disease: A Systematic Review

Richard W. Merritt, Edward D. Walker, Pamela L. C. Small, John R. Wallace, Paul D. R. Johnson, M. Eric Benbow, Daniel A. Boakye

PLoS Negl Trop Dis., Review article, published 14 Dezember 2010 | PMID: 21179505 | Free article  

9. Buruli ulcer prevalence and altitude, Benin.

Ghislain Emmanuel Sopoh, Roch Christian Johnson, Sévérin Y. Anagonou, Yves Thierry Barogui, Dodji Dossou, Jean Gabin Houézo, Delphin Mavingha Phanzu, Brice Hughes Tente, Wayne M. Meyers, Portaels.

Emerg Infect Dis., Letter, published January 2011 | PMID: 21192889 | Free article

10. Mycobacterium ulcerans DNA not detected in faecal samples from Buruli Ulcer patients: Results of a pilot study

Fred Sarfo, Caroline Lavender, Janet Fyfe, Paul Johnson, Timothy Stinear, Phillips, Richard
PLoS ONE, Research article, published 4 May 2011 | PMID: 21411582 | Free article

11. Interaction of Mycobacterium ulcerans with mosquito species: implications for transmission and trophic relationships.
Wallace, JR, Gordon, MC, Hartsell L, Mosi L, Benbow ME, Merritt RW, Small PL
Appl Environ Microbiol., Research Article, published 30 July 2010 | PMID: 20675453 | Free article   

12. Detection of Mycobacterium ulcerans in the environment predicts prevalence of Buruli ulcer in Benin.
Heather R. Williamson, Mark E. Benbow, Lindsay P. Campbell, Christian R. Johnson, Ghislain Sopoh, Yves Barogui, Richard W. Merritt, and Pamela L. C. Small
PLoS Negl Trop Dis., Research article, published 31 January 2012 | PMID: na | Free article 

Diagnosis

Fine needle aspiration (FNA) as sampling technique has been evaluated (13, 15) and cultivation from FNA and swab samples has been optimized (15). Cost efficient approaches for laboratory diagnosis based on optimized conventional techniques have been developed and evaluated (17, 18). Serology has been found inappropriate for the development of a simple point-of-care diagnostic test (14), leading to a re-focusing of our efforts on the development of antigen capture assays. Serology has been successfully validated in field studies as suitable tool for sero-epidemiology and led to the identification of a new endemic area in Ghana (16).

13. Fine-needle aspiration, an efficient sampling technique for bacteriological diagnosis of nonulcerative Buruli ulcer.

Eddyani M., Fraga A.G., Schmitt F., Uwizeye C., Fissette K., Johnson C., Aguiar J., Sopoh G., Barogui Y., Meyers W.M., Pedrosa J., Portaels F.
J Clin. Microbiol., Research article, published June 2009 | PMID: 19386847 | Free article 

14. Serological evaluation of Mycobacterium ulcerans antigens identified by comparative genomics.

Sacha J. Pidot, Jessica L. Porter, Laurent Marsollier, Annick Chauty, Florence Migot-Nabias, Cyril Badaut, Angèle Bénard, Marie-Therese Ruf, Torsten Seemann, Paul D. R. Johnson, John K. Davies, Grant A. Jenkin, Gerd Pluschke, Timothy P. Stinear

PLoS Negl Trop Dis., Research article, published 2 November 2010 | PMID: 21072233 | Free article

15. Isolation of Mycobacterium ulcerans from Swab and Fine-Needle-Aspiration Specimens.

Dorothy Yeboah-Manu, Emelia Danso, Kobina Ampah; Adwoa Asante-Poku, Zuliehatu Nakobu, Gerd Pluschke (2011)

Journal of Clinical Microbiology Bacteriology, article, published May 2011 | PMID: 21411582 | Free article  

16. Sero-epidemiology as a tool to screen populations for exposure to Mycobacterium ulcerans.
Dorothy Yeboah-Manu, Katharina Röltgen, William Opare, Kobina Asan-Ampah, Kwabena Quenin-Fosu, Adwoa Asante-Poku, Edwin Ampadu, Janet Fyfe, Kwadwo Koram, Collins Ahorlu and Gerd Pluschke
PloS Negl Trop Dis., Research Article, published January 2012 | PMID: 22253937 | Free article

17. Combining PCR with Microscopy to Reduce Costs of Laboratory Diagnosis of Buruli Ulcer
Dorothy Yeboah-Manu, Adwoa Asante-Poku, Kobina Asan-Ampah, Emelia Danso Edwin Ampadu, and Gerd Pluschke
Am J Trop Med Hyg, Research article, published June 2011 | Read abstract

18. Effects of decontamination, DNA extraction and amplification procedures on the molecular diagnosis of Mycobacterium ulcerans disease (Buruli ulcer)
Dissou Affolabi, N’Dira Sanoussi, Koen Vandelannoote, Mathieu Odoun, Frank Faïhun, Ghislain Sopoh, Séverin Anagonou, Françoise Portaels, Miriam Eddyani.
J Clin. Microbiol., Research article, published 11 January 2012 | PMID: 22259213 | Read abstract

Treatment

Clinical and histopathological studies have in particular focused on the investigation of patho-mechanisms responsible for paradoxical reactions during antibiotic treatment of BU (22, 21); mouse model studies have helped to interpret findings in patients (20). Results advocate for a role of wound debridement and surgical excision of necrotic tissue to reduce the length of hospital stays (19). Case studies have demonstrated that secondary lesions may heal spontaneously, speaking for the development of immune protection after successful antibiotic treatment (21). Current knowledge on treatment-associated inflammatory responses has been reviewed (19).

19. Immunosuppression and treatment-associated inflammatory response in patients with Mycobacterium ulcerans infection (Buruli ulcer).

Schütte D., Pluschke G.
Expert Opin Biol Ther., Review article, published in February 2009 | PMID: 19236249 | Read abstract  

20.  Chemotherapy associated changes of histopathological features of Mycobacterium ulcerans lesions in a Buruli ulcer mouse model.

Ruf MT, Schütte D, Chauffour A, Jarlier V, Ji B, Pluschke G.
Antimicrob Agents Chemother., Research article, published December 2011 | PMID: 22143518 | Read Abstract

21. Secondary Buruli ulcer skin lesions emerging several months after completion ofchemotherapy: paradoxical reaction or evidence for immune protection?
Ruf MT, Chauty A, Adeye A, Ardant MF, Koussemou H, Johnson RC, Pluschke G.
PLoS Negl Trop Dis., Research Article, published 2 August 2011 | PMID: 21829740 | Free article

22. Histopathological Changes and Clinical Responses of Buruli Ulcer Plaque Lesions during Chemotherapy: A Role for Surgical Removal of Necrotic Tissue?
Ruf MT, Sopoh GE, Brun LV, Dossou AD, Barogui YT, Johnson RC, Pluschke G.
PLoS Negl Trop Dis., Research Article, published 27 September 2011 | PMID: 21980547 | Free article

Socio-cultural studies

Family relationship, water contact and occurrence of BU in Benin has been studied (23). 

23. Family Relationship, Water Contact and Occurrence of Buruli Ulcer in Benin.

Ghislain Emmanuel Sopoh, Yves Thierry Barogui, Roch Christian Johnson, Ange Dodji Dossou, Michel Makoutodé, Sévérin Y. Anagonou, Luc Kestens, Françoise Portaels

PloS Negl Trop Dis., Research article, published 13 July 2010 | PMID: 20644620 | Free article